Sentence examples for media from confluent from inspiring English sources

Media from confluent cultures of BMDM was removed and replaced with the same media containing B. burgdorferi at various multiplicities of infection (MOIs).

For this purpose, the culture media from confluent HMEC-1 monolayers was replaced with calcium containing HBSS, and samples from the supernatant were collected at indicated time points, in the presence of POM-1, a highly potent ENTPDase inhibitor [6], [7].

The culturing media was also supplemented with 5% conditioned media from confluent WEHI3 cells, as source of interleukin-3.

Supernatant media from confluent DMS 79 cells were collected by centrifugation and used immediately or frozen at −20 °C.

In fact, others have reported increased HIF-1α expression when sub-confluent breast carcinoma cells were incubated with conditioned media from confluent cultures [ 12].

This fragment was determined using a selective enzyme-linked immunosorbent assay (ELISA; Quidel Corporation, Cederlane, Hornby, ON, Canada) in conditioned media from confluent Obs incubated in Ham F12/DMEM media containing 0.5% BSA.

The ability of galectin-1 endogenously produced from tumor cells in culture to protect against H2O2 induced EC apoptosis was assessed by adding conditioned media prepared from confluent cultures of the cancer cell lines to EAhy926 cell cultures (Fig.  7d).

The concentration of HGF in 48 h-conditioned media (50 μl) from confluent VSMCs grown in regular or osteogenic media was determined at specific time-points by ELISA (Quantikine HGF Immunoassay kit, R&D Systems), according to the manufacturer's instructions.

Serum-free conditioned media (SFCM) were collected from confluent cultures at 24 h, clarified by centrifugation and concentrated by 10-fold using centrifugal filter devices (Amicon, MWCO of 10 kDa).

(A) Representative western blot displays VEGF-C levels in conditioned media collected (24 hr) from confluent MDA231, MDASrc (pLKO.1), and MDASrc shEZR (shEZR).

Protein was extracted from confluent cells in fresh media as above cells were placed in a hypoxic chamber (Billups-Rothenburg, Del Mar, CA, USA) for 5 min at 20 l min−1 with 5% CO2/nitrogen gas mixture (BOC) and incubated at 37°C.