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The group of environmental bacteria enumerated depends on the media formulation and incubation conditions but are commonly known as heterotrophic plate count (HPC) bacteria; in Europe, this group is also referred to as autochthonous flora.
A specific growth media formulation and antibiotics were used in this study to achieve clinically simulated biofilm growth in vitro.
We hypothesized that undifferentiated hPSC growth rate, hEB formation, media formulation, and exposure to growth factors during cardiac differentiation could all be systematically optimized to improve cardiac differentiation.
These data supported the use of the Advanced media formulation and it was used throughout unless otherwise stated.
An important area for standardization is choice of media formulation and supplementary additives, including hormones, cofactors and antioxidants.
This work provides both a media formulation and a feeding strategy with a focus on nitrogen metabolism and regulation to support high-density algal culture without buffering.
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This study can be easily adapted for use with various cell types, media formulations, and potentially different bioreactor systems.
We will place particular emphasis upon the relationship between lactic acid productivity attained by various types of reactors, supports, media formulations, and lactic acid producing microorganisms.
All of the media formulations and test interpretations used have been described previously (Murray et al. 2003).
We have developed media formulations and growth conditions that support co-cultures of wild-type and the acetate-producing glgA1 strain of the Synechococcus sp. PCC 7002 and wild-type and lipid-accumulating sta6 mutant of C. reinhardtii (Additional file 2: Figure S1).
To improve expression and stability, several methodologies have been adopted, including methods based on media formulation, selective pressure and cell- or vector engineering.
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