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All culture media for primary cell culture were from Invitrogen.
8 Table 1 shows changes in antimicrobial supplements used in media for primary isolation of gonococci.
For the first time, quantification of volatile CPs in treatment solutions and culture media for primary cells becomes accessible by this validated method.
The swabs were incubated in sheep blood agar, saboured dextrose agar, macconkey agar, nutrient agar, and other selective media for primary isolation of the pathogens.
A proportion of cells from the prechemotherapy sample were seeded at a density of 2.5 5 × 10/mL in a minimum volume of 10 mL and cultured in Quantum 724 complete media for primary lymphocyte culture (QBL; PAA Laboratories Ltd., Yeovil, Somerset, UK) for 72 h prior to treatment to assess ex vivo damage.
Studies on the role of the IGF-IR in oligodendrocytes have been complicated by the fact that this receptor is also activated by insulin in the micromolar range (LeRoith et al., 1995), concentrations commonly used in chemically defined culture media for primary cells including embryonic neurons, OPCs and oligodendrocytes (Bottenstein et al., 1980; McCarthy and de Vellis, 1980).
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A copy of the full manifesto was posted on the document-sharing site Scribd by Gavin Sheridan, a reporter for Storyful, a news site that scours of social media sites for primary documents and eyewitness accounts of news events.
Transformed bacteria were incubated in auto-induced media 5052-ZYPprimaryprinvestigationgatiof of protein expression.
Samples were diluted with Endo media (1 50 for primary PNMEC cultures, 1 200 for immortalized PNMEC cultures), and aliquots (50 μl) were assayed according to the manufacturer's instructions.
Earlier this month, the tiny Hebridean island of Muck (population: 30) sent out a global appeal via social media for a primary teacher for its seven children.
To prepare the conditioned media for the primary cortical neuronal culture, Gibco™ Neurobasal™ Medium (Invitrogen, Cat No. 21103) supplemented with B27 (Invitrogen, Cat No. 17504-044) and L-Glutamine-Penicillin-Streptomycin solution Sigma-Aldrich, Cat No.. G6784) were used as basal culture media.
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