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Exact(5)
Cells were incubated in media for overnight after siRNA transfection, and then put under ER stress conditions.
Islets can then be transferred to a dish containing culture media for overnight incubation.
A single clone was picked and inoculated into 3 mL LB media for overnight growth.
In order to prepare the competent cells, a single E. coli colony was grown in 20 ml DYT media for overnight at 37 °C with 200 rpm.
To assess the role of androgen in the RSV effect on LNCaP (androgen-dependent) and C4-2B (androgen-independent, derived from the parental LNCaP) cells, the cells were grown in androgen-depleted (charcoal stripped) media and then either ethanol (vehicle) or R1881a (1 nM) was added to the media for overnight before mock infection or infection with RSV was performed.
Similar(55)
All these expressing strains were grown in SC-Ura media containing raffinose, instead of glucose, and that media replaced by galactose containing media for an overnight induction.
Further, each cleaned artery was placed in separate tube containing media at 4°C for overnight.
The calli were then blotted dry on sterile filter paper and transferred onto the PM media and left for overnight.
In brief, the recombinant S. cerevisiae cells were grown in 15 ml yeast synthetic drop-out media at 28 °C for overnight and harvested by centrifugation at 3000 rpm for 5 min.
Briefly, SH-SY5Y cells were seeded into 96-well plates at a density of 5 × 104 cells/well and incubated in media containing 10% FBS for overnight.
For total RNA extraction, cells were harvested 24 48 h post transfection and for Western blot, transfected cells were first incubated in complete media for 2 days, followed with incubation for overnight in condition media (medium without FBS).
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