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Selective media for isolation of fungi, actinomycetes and bacteria were prepared as described previously (Ko et al. 2010b).
Positive liquid cultures were sub-cultured on to LJ media for isolation of mycobacteria.
Several chromogenic media for isolation and identification of Candida species are available [ 12].
Unfortunately, the currently available enrichment and plating media for isolation of pathogenic strains of Y. enterocolitica are not selective enough to repress the background flora which increases the risk of false negative results.
Generally, definitive diagnosis of these infections is based on bacterial isolation, which can be difficult due to lack of suitable selective media for isolation of A. seminis and H. somni[ 5, 10].
Decontaminated specimens were inoculated into one slope each of Lowenstein-Jensen medium (L-J) containing glycerol and sodium pyruvate respectively and one vial of BACTEC 9000 MB media for isolation of M. tuberculosis.
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However, it is important to note that the media commonly used to isolate L. monocytogenes (particularly media used for isolation from food samples) contain acriflavine, a plasmid curing agent [ 61, 62].
Additionally, equine keratinocytes were not able to grow or grew poorly in Keratinocyte Serum free media (KSFM) with serum supplementation, a media designed for isolation of human and mouse keratinocytes (Table 1).
The media used for isolation of phylloplane fungi from leaf surfaces of gramineous plants, preservation of the isolated BP-degrading strains, and evaluation of production of BP-degrading enzyme were prepared as follows.
C. fetus is susceptible to cephalosporins, which are commonly included in media used for isolation of Campylobacter spp., making C. fetus isolation from feces unlikely.
The media used for isolation were SP-4 (4 ), DM-1 (5 ), and M-CMRL (6 ); all three supported the growth of Mycoplasma organisms.
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