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High density of free amino groups (-NH2) lying outwards the particle surface provides an excellent media for further chemical surface modification such as enzyme cross-linking with glutaraldehyde[5].
After three washes in KB solution, myocytes were resuspended in KB solution or in culture media for further experiments.
After 12 days, CD31+ cells (13.7±2.5%) were sorted by FACScan and maintained in EGM-2 media for further differentiation.
To evaluate different media for further use as basic growth medium, we applied a morphology score.
Biopsies were either placed into DMEM F/12 media for further isolation or fixed in Formalin.
The calli with a size of approximate 5 mm were selected and transferred onto fresh subculture media for further growth.
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The speakers also condemned the news media for furthering what they described as a calculated effort to discredit the woman, an immigrant from Guinea.
The description of a low-resource social media campaign may be a valuable tool for those wishing to embark on mass media campaigns for further tobacco control interventions.
Experiments involved incubating cells with various concentrations (0-2 μM) of doxorubicin for two hours followed a further incubation in fresh media for a further 24 hours.
Media was changed to fresh complete media for a further 24 h, and then 48 h following initial plating, TNF-α (150 ng/ml; PeproTech, London, UK), IL-1β (150 ng/ml; PeproTech), or IFN-γ (150 ng/ml; PeproTech) was added to cultures in fresh media for 48 h before fixation in 2% formaldehyde for 30 min followed by three washes in PBS.
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