Cells were then washed and re-suspended in culture media for analysis or in PBS for injection.
Finally, cells were pelleted, washed with DMEM/F-12+FBS or differentiation medium, and resuspended in 0.5 ml media for analysis.
To measure the polarized secretion of IL-6 and IL-8 quantitatively, 16HBE14o- monolayers were grown on a permeable support so that we could collect both apical and basolateral media for analysis.
Nasal swabs are rotated four times around the inside of the right nasal vestibule and then placed in Amies charcoal transport media for analysis.
To generate conditioned media for analysis by cytokine antibody array cell cultures were set up in suspension, on 6-cm plates coated with soft agar base layers (0.25 g/l glucose, 5% FBS medium containing 0.6% agar).
It has been difficult to get people to clean up and remove temporary data after analysis steps, but with a stricter policy, especially on storage quota, the users also have acquired new skills in big data management with compression tools and use of scratch media for analysis.
