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The "Access Hollywood" video, reported by the Washington Post, also led to a scurry among the news media for additional recordings of the GOP nominee, particularly the unaired footage from his years of hosting "The Apprentice" on NBC.
Cells were grown in serum-free media for additional 36 hr.
After 24 hours, cells were washed and incubated with fresh media for additional 48 hours.
The cells were then continuously cultured under normoxic or hypoxic conditions and induced in osteogenic or adipogenic media for additional 7 to 21 days.
Hippocampal neurons (DIV 18, 23) were treated with 20 µM and 50 µM NMDA (Sigma, Germany) for 5 min, washed and subsequently incubated in conditioned NB media for additional 25 minutes.
Four days later 10 µl BrdU labeling solution (1∶100) was added to the media for additional 18 h and BrdU incorporation into all genomic cellular DNA strands was assessed by ELISA following manufacturer's instructions Roche-Applied-Science Roche-Applied-Science1). Roche-Applied-Science, Cat
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25 mM HEPES was also included in the media used for Additional file 1: Figure S1, and we note that addition of the HEPES buffer reduced the respiratory growth rate of yeast.
Six hours after siRNA transfection, cells were resuspended in new culture media, incubated for additional 16 h, and then treated with TGZ.
Procuring the necessary licenses and media for any additional software.
For sorbitol assays, 3-day-old seedlings were transferred to either control or 270 mM sorbitol media for four additional days.
The cells were washed and re-cultured in media for an additional 2 days.
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