After the protests began, he'd taken the advice of the F.B.I., who'd suggested that officers in the area change their social-media profiles and passwords for added security.
The interior minister, while talking to the media, spoke about the need for added security for government personnel and emphasised the importance of better scrutiny of those who join the elite forces.
The retrovirus solution was mixed with the cells for 6 h, then certain amount of fresh 1640 media was added for reducing the toxcity of polybrene.
Cells were incubated in DM for 48 h and washed with PBS, and fresh RM media were added for another 48 h with or without experimental factors.
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Acridine orange (1 μg/mL in serum-free media) was added for 15 min prior to gentle washing with PBS.
After 20 h, cells were washed, and fresh media were added for 20 h before performing a second round of infection.
After 30 min, 100 μM puromycin with 200 μM emetin in L15 media was added for 10 min. Cultured neurons were then washed with L15 and immediately fixed with 4%PFA/30%% sucrose for 30 min. FISH was performed as described in Lyles et al. (2006) with slight modifications.
Seedlings were grown in hydroponic medium containing 250 μM Pi for 30 d before transfer to media lacking added Pi for 14 d to maximise AtPHT1 transcript abundance.
Briefly, after [H] cholesterol labelling the cells, media containing HDL (20 µg/mL) or media alone was added for up to 4 h, filtered and counted by liquid scintillation.
