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The co-expression of ustR and other genes of this cluster was also detected in CDEm and YPM liquid media (data not shown).
Samples were observed for 21 days incubation in three different minimal salt media, where one medium M7 showed somewhat better growth than rest two media (data not shown).
Both phenotypes were flocculent when grown at either 30°C or 42°C and, in both cases, flocculation was abrogated when Ca2+ was absent from the culture media (data not shown).
At increased concentrations, the MNPs became attached to either the cell membrane or the bottom of the plate, appearing as a brown colour in the well (even after five washes with fresh culture media) (data not shown).
by 8% compared to solid media (data not shown).
The strain was not growth defective in either LB or M9 minimal media (data not shown).
Down regulation of Cx43 was observed by lower incidence of LY uptake in a Ca++ free media (data not shown).
Furthermore, we observed that peptides did not inhibit the growth of E2348/69 in LB media (data not shown).
No significant differences were found between the CFU counts in TSA and in the two selective media (data not shown).
However, this could be due to faster growth of these microorganisms on the given media (data not shown).
MF201, MF213 and MF214 exhibited no growth defects either in liquid media (Figure S1) or on solid media (data not shown).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com