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Therefore, additional samples were placed inside a standard laboratory CO2 incubator filled with culture media (Ctrl) and under normal culture conditions (Inc).
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Conditioned cell media from ctrl, LOX-expressing, or LOXL2-expressing cells were concentrated as described, and 100 μl of either ctrl supernatant or of a mix of 50 μl LOX supernatant and 50 μl LOXL2 supernatant was injected intraperitoneally every day for the indicated time.
The outer and inner-media of CTRL-ATA, BAV-ATAA and TAV-ATAA were imaged using multi-photon microscopy in the Z-RAD and Θ-RAD planes to observe collagen and elastin.
Next, wells were immersed with RM (CTRL) and experimental media for another 48 h (percentages of control value).
To confirm the autocrine secretion of mitogenic ligands, serum-starved HB4a and C3.6 cells were treated with conditioned media (CM) from serum-starved (Ctrl), EGF-, or HRG β1-treated cells, and activation of MAPK signalling monitored after 15 min by immunoblotting with a phosphospecific antibody.
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Exosomes isolated from the culture media of cells expressing cel-miR-238 (exo-m238) or from control cells (exo-Ctrl) were added to the culture media of recipient naïve MIN6B1 cells.
Ctrl C, Ctrl V from last year: the LFP of course … and someone really needs to invent an irony font for that 'P'.
Clearly, Ctrl C and Ctrl V (or command C and command V for the Mac users among you) have become dangerous weapons.
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Ctrl control.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com