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To test this hypothesis in vitro, NCM's were transfected with adenovirus expressing LacZ, dnMSTAT, and MSTAT as previously described, then stressed with PE or media control for 15 minutes.
To determine the effect of pfGPI on monocyte C5aR (CD88) expression, PBMCs were incubated as above with pfGPI or media control for 24 or 48 hours, and C5aR and CD14 expression (used to define monocytes) was determined by flow cytometry.
(D ) DCs treated with 0.5 μM rapamycin or media control for 18 hr prior to treatment with 0.5 μM FK506 or media control for 1 hr and washed extensively.
Monocyte derived DC were treated with 0.5 µM Rapamycin overnight followed by 0.5 µM FK506 or media control for 2 hr and washed extensively.
After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with TGFβ1 10 ng/ml (Peprotec, Canada), GHK 10 nM (Sigma, Canada) or growth media control for a further 48 h.
To examine whether MTX might preferentially alter PpIX accumulation depending upon whether the prodrug was in the form of free ALA or one of its esters, LNCaP cells were pretreated with MTX or media (control) for 72 h, and then exposed to ALA, m-ALA, or h-ALA for 4 h.
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Quality control procedures included positive and negative media controls for all target organisms and temperature control of all incubators with continuously operating digital temperature displays and additional electronic devices recording minimum and maximum temperatures throughout the incubation period.
The order, from Li Changchun, China's top media control official, called for a shift away from covering events in China.
At day 14, cerebral organoids (controls or PD173074 treated) were treated with 100 µM BrdU for 2 h (pulse phase), washed with DMEM-F12 three times and then incubated with media (control or PD173074) for an additional 4 days (chase phase).
Thus, experiments attempting to measure the activity of extracellular TG2 in cell culture require a conditioned media control to account for TG2 released into the media due to cell lysis during the experiment.
DC were cultured with 0.5 μM FK506 (Astellas Pharmaceuticals, Killorglin, Ireland) or media control at 37°C for various intervals and washed.
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