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Synovial membrane harvested from either porcine or human knee joints was enzymatically digested, and synovial MSCs were isolated, and then expanded in growth media containing virus- and prion-free fetal bovine serum.
The media containing virus was filtered with 0.45 µm filter.
Transduction was done with 1 mL of media containing virus.
After 36 48 hr, collect media containing virus and add fresh media.
Centrifuge the media containing virus at 150 × g for 5 min to pellet any packaging cells that were collected during harvesting.
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Tissue culture media were refreshed 15 h after transfection and media containing viruses were harvested 45 h after transfection.
Forty-eight hours post transfection, the media containing the virus was collected, filtered, and centrifuged at 70,952 × g for 2 h at 4 °C.
After the infection, the media containing the virus was removed and replaced by fresh infection media, followed by incubation for the indicated times at 37°C.
After 24 hours the media containing the virus was removed and polybrene was added to 4 μg/mL.
Media was removed and replaced with media containing HIV virus-like particles containing β-lactamase as described [20].
Cloacal swabs were collected randomly in sterile tubes containing virus transport media (VTM) (MART; Remel, Inc., Lenexa, KS, USA) for virus isolation and identification.
More suggestions(15)
media containing ampicillin
media containing ligand
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media containing vancomycin
media containing yeast
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