Frozen bacterial strains were streaked for single colonies and one single colony was used for inoculation of liquid TY medium containing tryptone (Hi-Media, Mumbai, India) 8 g/L, yeast extract (Hi-Media) 5 g/L, and sodium chloride 5 g/L.
Clonal recombinant cultures were established by colony purification on a solid complex medium containing tryptone (0.2% w/v).
Biofilms were cultured using a modified Luria-Bertani broth containing tryptone 1.0 g L−1, yeast extract 0.5 g L−1, NaCl 10.0 g L−1.
For the cytotoxicity assays, 100 μL bacterial culture was seeded in 3 mL DMEM (Gibco, Rockville, MD) or DMEM containing tryptone 1% (DMEM-tryptone) and cultured at 37°C with shaking at 200 rpm until the exponential phase.
Therefore, we investigated cytotoxicity of pet+ aEPEC and EAEC strains in a comparative study using DMEM both containing tryptone and not.
Samples (1 mL) of overnight cultures (cultured with or without 0.5 mM IPTG) were mixed into a soft agar overlay containing 3 mL of media containing 10 g/L tryptone, 1 g/L yeast extract, 8 g/L NaCl, 8 g/L agar, the appropriate antibiotics, and 1 mM IPTG.
A 24-hour-old culture 0.5% (w/v) inoculum was taken and inoculated into a 250 mL Erlenmeyer flask containing 100 mL sterile production media containing raffinose 25, tryptone 10, K2HPO4 10, MgSO4·7H2O 1, and FeSO4·7H2O 1 in g l−1 (pH 7.0).
Batch culture led to a lipase production of 26 450 U ml−1 in a media containing olive oil and tryptone as carbon and nitrogen sources.
In brief, the H. salinarum colonies were cultured in 6 L tryptone media containing 4 M NaCl under high oxygen tension and continuous illumination with 200 W LED lamp for almost 192 h at 39 °C.
