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Media were replaced 24 h later with complete media containing the indicated concentrations of tamoxifen (Sigma).
To induce apoptosis, cells were treated for the indicated period of time in fresh media containing the reagents indicated in the figure's legends.
Forty-eight hours post transfection, the media containing the virus was collected, filtered, and centrifuged at 70,952 × g for 2 h at 4 °C.
The scala media, containing the organ of Corti and filled with endolymph, cannot be visualised separately with current imaging systems.
Cells were incubated with media containing the PEGylated nanoparticles at concentrations ranging from 5 100 μg/mL.
Therefore, A. fumigatus AFGRD105 demonstrated enhanced production of mycelial biomass and melanin when cultured in media containing the unsubstituted SDA.
Media containing the secreted protein was harvested 6 7 days post transfection and purified using protein A chromatography (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
Cells cultured under these serum conditions were exposed to NPs suspended in media containing the respective serum concentrations and visualized through HS imaging.
Thus we utilized a minimal media containing the N2 supplement and EGF alone.
Following 90 minute incubation, media was replaced with fresh media containing the drug used.
Following a 30 minute pre-incubation, media was removed and replaced with serum-free media containing the inhibitor or vehicle.
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