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Cultures were grown in rich (YPD) or synthetic media containing standard ingredients and 2% glucose (SD medium), or 2% raffinose (SR medium), or 2% galactose (SG medium), or 2% raffinose + 2% galactose (SRG medium).
Synthetic media containing standard ingredients and 2% glucose (SD medium, non-inducing), 2% raffinose (SR medium) or 2% galactose (SG medium, inducing) were used throughout.
For phenotypic observation, ten seedlings were manually transferred to test media containing standard medium alone or also including the indicated additives, and were examined using a dissecting microscope (Leica EZ4 HD).
Yeast cultures were grown in rich (YPD) or synthetic media containing standard ingredients and 2% glucose (SD medium), 2% raffinose (SR medium), or 2% raffinose + 2% galactose (SRG medium).
Moreover, PaGlo1/PaGlo2_OEx strains are not long-lived on media containing standard glucose concentrations suggesting a tight correlation between the efficiency and capacity to remove MG within the cell, the level of available glucose and lifespan.
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Eclosing virgin females and males were collected into different bottles containing standard media from day 9 to day 10.
Animals were then shifted to 25°C for 8 hr, returned to 15°C for 12 hr, and then transferred to plates containing standard media for 2 3 days.
Flies were cultured from egg stage on 94 × 25 mm polystyrene vials containing standard media supplemented with each test compound as stated previously.
For cell binding studies, Huh-7 hepatocytes (a gift from Srikanta Dash, Tulane Health Sciences Center) were expanded in 6 well plates containing standard media at 37°C with 5% (v/v) CO2.
Drosophila were collected over a 24 hour period and aged in vials containing standard Drosophila media (40 50 Drosophila/vial; same sex) and transferred every 3 6 days to fresh Drosophila media.
For the three-camera tracking assays, vials containing standard fly media stained with blue food coloring (Kroger brand) were used, as the blue colored background was required for efficient tracking.
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