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While media containing serum can also induce C. albicans filamentation, we found that filamentation genes were more highly induced in the phagocytosed C. albicans subpopulation (Supplementary Data 2; Supplementary Figure 2B).
After 120 h, all media were aspirated and replaced with 60 μL of culture media containing serum to each well.
Compared to media containing serum or undefined components, PRIME-XV's serum-free formula is designed to increase experimental reproducibility and culture consistency to maximize the use of these cells.
The supernatant was poured into 20 x vol of extraction media containing serum to inhibit trypsin activity.
Chase was performed with media containing serum (20% dialyzed FCS, 20 mM Hepes pH 7.2, 50 mM deferoxamine, 2 mg/ml holo-transferrin) at 37°C.
Medium fractions prepared from media containing serum, but not conditioned by HepG2 cells, showed no activity within our bioassay suggesting that detected activity was derived from the HepG2 cells (data not shown).
Similar(20)
Cells were collected by trypsinization and resuspended as single cells in cancer stem cell media containing serum-free DMEM/F12 (Invitrogen) supplemented with 100 units/mL B27 (Gibco), 10 µg/mL Insulin (Sigma), 20 ng/mL EGF (Sapphire Bioscience), and 20 ng/mL bFGF (Sapphire Bioscience).
We have shown previously that cancer cells responded similarly to micromolar concentrations of MF when cultured in media without phenol red and charcoal-extracted fetal bovine serum or media containing unextracted serum and having phenol red [ 13].
3 × 10 serum-starved cells were loaded in media containing 0.1% serum in the insert while the well was filled with media containing 10% serum.
There was a statistically significant correlation between the tetanus specific stimulation index (S.I). of the non-cryopreserved PBMC and SI obtained from cells frozen with media containing human serum albumin as compared to other additives such as dextran or fetal bovine serum.
Pooled peripheral blood mononuclear cells (PMBCs) from healthy volunteers were incubated for 20 hrs with media containing 30%% serum from either trauma patients or healthy volunteers.
More suggestions(15)
medium containing serum
media incorporating serum
media containing erlotinib
media containing glucose
media containing growth
media containing virus
media containing ampicillin
media containing ligand
media containing cellulose
media containing galactose
media containing compound
media containing hemin
media containing glycerol
media containing ammonium
media containing lactate
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