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This can be evaluated by growth tests on media containing lactate as the sole carbon source, in which swf1Δ cells cannot grow.
Figure 2A shows that, when driven from a TPI1 promoter, which normally leads to moderate overexpression of proteins, neither Erf2, Pfa3 nor Pfa4 can complement swf1Δ growth deficiency in media containing lactate as a carbon source.
We had demonstrated before that on complex media containing lactate Psd1p became paramount as a PE synthesizing enzyme, and even exogenous supply of Etn could not restore the cellular and mitochondrial levels of PE [31].
To make lipid metabolic conditions for the yeast as stringent as possible, especially with respect to the requirement for PE, we cultivated cells on minimal media containing lactate as a non-fermentable carbon source.
To further the understanding of molecular pathways responding to the heavy metal chromium VI) [Cr VI ], the proteome of aerobically grown, Cr VI -stressed P. putida strain F1 was Cr VI -stressedithin the context of two disP.rate nutritional environments: rich (LB) media and minimal (M9L) media containing lactate as the sole carbon source.
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Solutions containing lactate were found to yield spherical calcite crystals.
Lactate/lactic acid has been considered as a biofuel for enzymatic biofuel cells, but only with single enzyme bioanodes containing lactate dehydrogenase.
Increased relative growth rates were particularly pronounced for acidic conditions and media containing osmolytes, but particularly for wells containing sodium lactate [6].
Therefore, we checked the amount of secreted lactate by C. cellulovorans cultivated in media containing NaH13CO3 (Figure 4a).
When yeast cells are cultured in media containing a nonfermentable carbon source, such as glycerol or lactate, they shift from anaerobic to aerobic respiration.
Our results demonstrate that when C. cellulovorans was cultivated in media containing NaH13CO3, the relative fractions of pyruvate + oxaloacetate (OAA), lactate, fumarate, and malate inside (Figure 2a) and outside (Figure 2b) cells were significantly higher than those from C. cellulovorans cultivated in media containing NaHCO3.
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