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At 4 h post-transfection, the transfection media containing nanocomplexes were replaced with FBS-containing media.
By comparing the migration and cell growth of BMSCs stimulated with conditioned media from co-cultures of WT cells and IL-6 KO cells, it was found that only conditioned media containing IL-6 stimulated the migration and increased the cell numbers of BMSCs, while conditioned media lacking IL-6 could not.
Our data obtained with hippocampal tissue are further supported by a recent in vitro study showing that conditioned media containing APPsα can to some extent (by about 50%) restore spine density deficits of cultured APP-KO neurons [ 63].
For E2-containing media, E2 was added to standard media to create a media containing 1 μM E2.
media was replaced with media containing compounds.
Finally, either media or media containing chemotherapeutic was added.
1) control; 2) algal growth media containing 5% of A. fumigatus/ TWS media; 3) algal growth media containing 20% A. fumigatus/ TWS media.
(A) BG11 growth media containing 5% of A. fumigatus /TWS media; B) BG11 growth media containing 20% A. fumigatus /TWS media.
On day 3, culture media was changed to either serum-free media or media containing 1% fetal bovine serum.
The media used were standard synthetic media or rich media containing 2% D-glucose or 2% D-galactose.
After 7 d, the media was exchanged for fresh MS media containing the compound of interest.
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