Sentence examples for media containing increasing from inspiring English sources

Z. mobilis strains with increased tolerance toward furfural and acetic acid have been obtained by sequential transfers of cell cultures to synthetic media containing increasing concentrations of inhibitors (Shui et al. 2015).

Following isolation and transfer of hygromycin-resistant mycelial slices to media containing increasing concentrations of hygromycin B, we found that transformants were able to propagate in the presence of hygromycin B concentrations as high as 300 μg/ml (Figure 3c, bottom panel).

These new cultures were used to inoculate media containing increasing concentrations of TET that ranged from that from which the inoculae were prepared to higher concentrations and incubated at 37°C until evidence of full growth was present.

To test this hypothesis, PC12 cells lines expressing wild type human ZnT3 and tyrosine mutants were cultured in media containing increasing concentrations of ZnSO4 during 24 h (Fig. 5E).

After four days, the protoplasts were transferred to media containing increasing concentrations of Latrunculin B. Two days after incubation in Latrunculin B, whole plants were imaged to determine plant area and solidity (see Methods).

Six hours later, transfection media was removed and replaced with regular growth media or media containing increasing concentrations of cisplatin (0uM, 5uM, and 10uM).

A beneficial effect on lifespan is observed when both PaGlo1 and PaGlo2 are overexpressed and the corresponding strains are grown on media containing increased glucose concentrations.

Circular plasmids were transformed to P. pastoris GS115 and the transformation suspensions were plated on rich agar media with glucose or minimal agar media with methanol containing increasing concentrations of Zeocin to directly select for the transformants containing the P AOX 1 promoter variants leading to stimulated expression compared to that of the wild-type.

For transient transfection experiments, 1.7 × 10 HEK293 T-Rex cells were seeded in 12-well dishes and transfected with a TNFα expression plasmid (Maifeld et al., 2011) using Lipofectamine 2000 (Invitrogen) for 2 hr, after which the culture media was changed to one containing increasing concentrations of CT8.

N2a neuroblastoma cells were incubated in serum-supplemented or serum-free media and the serum-free medium contained increasing concentrations of CysC.

We applied a similar procedure to engineering n-butanol tolerance – E. coli cells carrying pQ-lib were serially transferred in media containing gradually increased n-butanol concentrations.