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The exposure follows the model described by Krocova et al. (2000): elicited peritoneal macrophages were placed into the wells of 96-well microtiter plates (Corning Inc., Corning, NY), allowed to adhere for 4 hr, and then exposed to cRPMI-1640 media containing DU (10, 50, 100, 200, 500, and 1,000 μM) or an equal amount of medium with no DU added.
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Cells were mounted with Vectashield mounting media containing DAPI Vector Laboratoriess, CA, USA).
After incubation cells were washed and then coverslips added with mounting media containing DAPI.
Herein, we investigate a condition in which firstly GO is dispersed in acidic media containing APS.
Briefly, cells cultured in 100 × 20 mm tissue culture dishes at 90% confluence were treated with media containing DMSO or crizotinib (doses used as described in the text) for 6 hours.
Then the media containing MTT was removed, leaving the precipitate.
The measured MnP activity was much lower in the media containing HA compared to the control jars (media without HA).
To observe the morphology, the cells were seeded at a concentration of 1 × 105/ml in a 10-ml petri dish and incubated for 3 days with a media containing GM or GF at a concentration of 0.2 mg/ml.
After incubation in media containing MTS reagents, scaffolds were removed from the CellCrown™ and washed in PBS.
Upon washes in TBST, the sections were mounted in mounting media containing DAPI (Vectashield) and visualized with Zeiss Axiovert Microscope.
Slides were mounted in media containing DAPI and images were acquired on a Leica DMI 6000 b automated inverted microscope.
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