Sentence examples for media containing calcium from inspiring English sources
In this study, pH-sensitive blended polymeric beads were prepared by ionic gelation of mixed alginate and N,O-carboxymethyl chitosan (NOCC) solutions in aqueous media containing calcium chloride.
However, T3S phenotypes are usually determined by culturing bacteria in media containing calcium chelators [ 4, 5, 12, 26].
For the following 42 h, PTH 1 34 was removed, and the cells were cultured in cell-culture media containing calcium chloride alone.
Following completion of calcium flux measurements, media containing calcium dye were aspirated and cardiomyocytes were washed with 80 μL of KRBG (pH 7.5) before 15 μL of stimulation buffer (KRBG containing 800 nM IBMX) was added (Table 1 ).
When the PPP component of the blood is subsequently combined with any culture media containing calcium ions, fibrinogenesis can resume, resulting in a stable hydrogel, formed by the donor's native clotting system, incorporating the nutrient rich culture media.
This is in contrast to Dahm et al [ 16] who found media containing a calcium concentration of 0.6 mM optimal in their study, as with higher calcium they found early differentiation and loss of cell proliferation.
Physical and proteolytic tissue separation in combination with media containing a calcium concentration of 0.6 mM in combination with additional media supplements proved optimal for proliferation and subculture of equine lamellar keratinocytes on collagen coated substratum.
Although the in vivo signals which trigger T3S are poorly understood, production and secretion of the effectors can be induced in vitro by growing the bacteria in media containing a calcium chelator such as nitrilotriacetic acid (NTA) [ 9].
EA uptake by HeLa cells in calcium-free media was significantly reduced compared with medium containing calcium (Fig. 5A).
At 30 minutes post-infection, cells were washed twice with PBS containing calcium and magnesium and incubated in fresh media containing 1 mM IPTG and 50 µg/mL gentamicin.
Media was then aspirated and slips were washed three times with PBS containing calcium and magnesium (PBS++).
