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The bottom wells were filled with serum-free media, serum-free media containing 10 ng ml−1 of G-CSF or serum-containing media (positive control).
The resulting strains were plated on media containing 5-fluoroorotic acid (5-FOA), which selects for cells having lost the URA3-containing plasmid carrying the PAM16 gene.
Fibroblast proliferation was suppressed in media containing CSNPs.
Utilization of various carbon sources was assessed on a minimal media containing M9 salts.
Cells were incubated in media containing 10-μg/mL S- and L-MWCNTs for up to 24 h.
The highest stimulation was observed in media containing Pure-PC, that prolonged L. acidophilus growth up to 72 h.
Results indicated that hFOB 1.19 cells proliferate and differentiate well in treatment media containing CNTs-HA and graphene-HA.
Mince washed with media containing MgCl2 had a slightly higher pH than those washed with media comprising CaCl2.
White and gray bars represent the ratios of each metabolite in media containing NaHCO3, and NaH13CO3, respectively.
Cell proliferation in media containing PEG-immobilized CSNPs (PCSNPs) was almost the same as that in the control culture dish.
Next, Ura- derivatives of the transformants were selected on media containing 5-fluoroorotic acid (Boeke et al. 1987).
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