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The host cells were then incubated on LB liquid media containing ampicillin.
Protein expression was performed in LB media containing ampicillin (100 µg/ml) and chloramphenicol (50 µg/ml).
The PCR product, thus obtained was purified, digested with DpnI restriction enzyme to cleave the methylated template DNA, transformed into E. coli DH5α strain and plated on LB agar media containing ampicillin (50 µg/ml).
DNA fragments were ligated into pGEM-T Easy vectors (Promega) and transformed into competent E. coli which were grown on selective media containing ampicillin.
The individual clones were picked up and grown in deep well plates in 2× YT media containing ampicillin (75 μg/ml) at 37°C for 16-18 houndernder shaking at 250 rpm.
For protein production, E. coli cells were grown at 37 °C in 1 L LB media containing ampicillin and 1 % glucose to an OD600 of approximately 0.5, induced with 1 mM IPTG and grown for 5 h at 37 °C.
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All culture media contained ampicillin at a final concentrations of 0.04%.
All four media contained ampicillin (60 μg/ ml) to eliminate contamination by bacteria.
Mixed cultures of A. apis strains ARSEF 7405 and 7406 [ 7] were grown at 35° C in liquid or solid YGPS growth media [ 2] containing ampicillin (100 μg/ml) and streptomycin (12 μg/ml).
Five recombinant bacterial colonies (white) per isolated band were picked from the plate containing ampicillin and X-gal as selective media and cultured overnight at 37°C.
E.coli DH5α/pXJ79cu and E.coli DH5α/pXJ80cu were cultured in LB containing ampicillin and chloramphenicol, respectively, at 30 °C.
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media containing virus
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