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Media was replaced with fresh media containing a range of doses of DCA (0, 10, 20, and 50 m M).
Media containing a chemoattractant (10% FBS) or serum-free media + 0.1% BSA (negative control) was added to the bottom chamber of the Transwell plates.
Surface tension, surface shear and dilatational viscosities were measured in a variety of media containing a surfactant.
A constitutive relation for multiphase porous media containing a phase-change constituent is developed based on a periodic representative unit cell, microstructure superposition technique, and homogenization scheme.
The challenge now lies in understanding complex real-life systems consisting of mixed multicomponent solids in media containing a variety of organic and inorganic species.
To verify the new model, examples involving deformable porous media containing a single and two sets of fractures were designed, showing good accuracy.
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In part II of the paper, Antiplane shear deformation of bi-material circular media containing an interfacial edge crack is considered.
Cells treated with media containing an equivalent amount of DMSO served as a negative control.
As a control, cells were incubated with media containing an equivalent volume of vehicle (DMSO) for 6 h.
Both organisms are soil-dwelling, non-pathogenic saprophytes, but have been differentiated by the ability to produce pigment on nutrient media containing an organic nitrogen source [13].
At the start of each experiment, 100 µl serum free media or 100 µl serum free media containing an antagonist at the final required concentration was added to the wells and the cells incubated for 30 minutes at 37°C.
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