The ball mill used was a Dynomill and the grinding media consisted of zirconium oxide beads.
Baseline glucose control media consisted of assay medium that was incubated for 4 h at 37 °C without cells.
Plating media consisted of 850 μl of Neurobasal supplemented with 10% horse serum (HyClone), 0.5 mM GlutaMAX (Invitrogen) and 2% B27 (Invitrogen).
Growth media consisted of 850 μl of DMEM (Invitrogen) supplemented with 10% horse serum, 0.5 mM GlutaMAX and 1 mM sodium pyruvate (Invitrogen).
The optimum media consisted of 5% (w/v) yeast extract; 18.9% (w/v) soy peptone; 5% (w/v) glycerol and 0.06% (w/v) K2HPO4.
Isolated cells were then cultured in growth media consisted of D-media (Formula No. 78-5470EF, GiBRL BRL) containing EGM-2 SingleQuot and 10% FBS (Gibco BRL) at 37 °C in humidified atmosphere of 5% CO2 in air.
Porous media consisted of homogeneous wettability sintered glass, and mixed wettability reservoir rock samples: a sandstone from the North Sea and a carbonate from the Middle East (preserved state).
Laboratory tests for biofiltration treatment of RSCs were performed in order to compare the properties of two filter media, consisted of a mature compost with yard waste (YW) and a mixture of mature compost with sewage sludge and yard waste (SS/YW).
Porous PbO2 deposits obtained from both media consisted of the same mixture of α- and β-phases, although the corresponding compact deposits had significantly different structures (pure α-PbO2 from methanesulfonic acid, a mixture of α- and β- PbO2 from perchloric acid).
Air liquid interface (ALI) media consisted of the PneumaCult kit purchased from Stem Cell Technologies (Vancouver Canada).
Submerged cell culture media consisted of Bronchial Epithelial Cell Growth Medium (BEGM) with antibiotics from Lonza (Walkersville, MD).
