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NP agarose constructs were cultured to functional maturity and treated with combinations of IL-1β and media conditioned with IL-1ra released from microspheres at intervals for up to 20 days.
We thus assayed by qPCR the above selected miRNAs in culture media conditioned with a panel of HCC cell lines, including HepG2, Hep3B, PLC/PRF/5 and MHCC-97H.
We hypothesized that a circulating factor was mediating our observed changes in mitochondria; therefore, we incubated human primary dermal fibroblasts in media conditioned with 10% human serum acquired from young SED and ACT individuals prior to and following acute exercise.
The further analysis of media conditioned with human mammary epithelial cells revealed the presence of the active as opposed to latent form of TGF-β, thus implying a direct link between soy isoflavones and the TGF-β signalling pathway.
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PC12 cells displayed enhanced neurite outgrowth in media conditioned by conduits with transdifferentiated MSCs.
To determine role of paracrine signaling, we cultured pure mESC-CMs within miniature tissue "micro-patches" supplemented with media conditioned by adult or fetal CFs.
We thus treated HMEC1 with media conditioned by control or vGPCR-expressing cells and assessed the activation of TSC kinases by these supernatants.
Rich media conditions may better reflect the situation of the pathogens in the host (like e.g. in the gut), in comparison to minimal media conditions with clearly defined carbon sources for which flux balance analyses can be well adapted.
To test the predictions of iSR432, we simulated growth of C. thermocellum by applying FBA, assuming minimal media conditions with one of two possible carbon sources (cellobiose or fructose).
Media were conditioned with IL-1ra released from microspheres at intervals up to 20 days.
After a 24 h efflux period, we observed a significant enhancement of hBMVEC Fe-efflux when the efflux media was conditioned with EC/-/C6 as compared to medium that was conditioned by either cell type alone.
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