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A 2.5-fold increase in enzyme activity with 1.3-fold decrease in the cost of enzyme production (in terms of media components) was achieved by using different technological combinations.
The flux through the exchange reactions for these media components was constrained to νmin ≥ -100 mmol/gDW/h and νmax ≤ 1000 mmol/gDW/h, where a negative flux indicates the uptake rate of the metabolite.
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Yeast media components were supplied by Sigma Aldrich.
Other common chemicals, biochemical, and media components were purchased from standard commercial sources.
For efficient bioethanol production, four major media components were statistically optimized by response surface methodology (RS M.
The concentration of four media components were optimized by using central composite design of response surface methodology.
From a total of fifteen, four important natural media components were screened based on biomass, total carotenoids and astaxanthin production.
The concentrations of these four media components were optimized by using central composite rotatable design (CCRD) of RSM.
In order to achieve this, combinations of media components were formulated by employing statistical tools such as Plackett Burman analysis and central composite rotatable design (CCRD).
Other media components were kept unchanged.
All media components were supplied by DifcoTM (BBL/Difco Laboratories).
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