For bacterial BAL culture, we used three different media: chocolate Poly ViteX agar, MacConkey agar and blood agar.
Cultures were obtained with swabs (BBLtm CultureSwab™, made by Copan for Becton, Dickinson and Company, Sparks, MD, USA) and plated on three different growth media: chocolate agar, trypticase soy agar with 5% sheep blood, and mannitol salt agar (Remel Products, Lenexa, KS, USA).
His media mixing (chocolate on canvas, anyone?) has been shown in exhibitions across the globe, and this fall he has openings in Paris, Athens and Tokyo and a retrospective in Madrid.
All corneal cultures in the current study were obtained via corneal scraping along the involved suture site and were plated directly onto several different culture media, including chocolate agar, 5% sheep blood agar, and Sabouraud agar.
But then why not work with a more aesthetically pleasing food media, like chocolate?
Chocolate connoisseurs are trending and the media says that chocolate is a health food.
Please feel free to check out my favorite productivity tools, social media, books and chocolate on Pinterest.
At best, growth may be obtained on rich, non-selective media (blood or chocolate agar) incubated in a micro-aerobic (5% O2) atmosphere at 35°C [ 11].
Efforts to culture the organism from biopsy specimens were again unsuccessful (different solid and liquid media, blood or chocolate agar, guinea pig, Balb/c mice).
At the same time, subcultures were made during 3 successive days on enriched and selective media including blood, chocolate, MacConkey, and mannitol salt agar plates and examined for growth after 24 48 hours of incubation.
Lymph node aspirate from the patient cultured in horse blood and in blood cysteine and chocolate media with and without supplement (BBL IsoVitaleX Enrichment; Becton, Dickinson and Co., Sparks, MD, USA) produced no growth after 2 weeks of incubation (37°C, 5% CO2); the control strain of F. tularensis live vaccine strain grew within 3 days.
