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Media were replaced every 4 days and media changes were performed in normoxic conditions.
All cells were cultured as described27,28, were maintained at 37 °C in a humidified atmosphere of 5% carbon dioxide with biweekly media changes, were drawn from stored stock cells, and replenished on a standardized periodic basis and were routinely monitored for Mycoplasma (PlasmoTest™, InvivoGen, San Diego, CA), at least every 3 months.
Half media changes were done every other day for the duration of EB culture.
Purified IFNα (PBL Biomedical Laboratories) was used across the range of 5 5000 U/ml, with addition at the time of viral infection, and replaced whenever media changes were performed.
Media changes were performed every two days.
Subsequent media changes were performed thrice a week.
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Media collected at each media change were also used to analyze the PG content released into media on a cumulative basis.
The potency of multilamellar decitabine-loaded liposomes and pure decitabine without media change were lower than those with the media change.
50% media change was performed every 24 48 h with differentiation media.
Media change was ensured every 48 h.
Media change was performed every 4 days.
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