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The effects of variations in the number of pulses (5 50 pulses), electric field strength (15 30 kV/cm), temperature (0 60°C) and media bases (water and skim milk) on the inactivation of L. monocytogenes were examined.
In order to optimize the media for F420 production, the ZY component of ZYM 5052 media was replaced by commonly used media bases including 2× ZY, YT (0.8% tryptone, 0.5% yeast extract and 42.77 mM NaCl), TB (1.2% tryptone, 2.4% yeast extract and 0.4% glycerol), SOB (2% tryptone, 0.5% yeast extract, 8.56 mM NaCl, 2.5 mM KCl and 10 mM MgCl2) and SOC (SOB with 20 mM glucose).
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