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Control differentiated cells were injected with new growth media at equal amounts.
To match the effective strength of PtetA to that of Ptrc, anhydrotetracycline, which inhibits Tet repressor, was added to the three media at equal concentrations of 0.5 µg/l.
After 24 hr, cells were resuspended in 20% conditioned media at equal densities and supernatant samples at baseline, 24, and 48 hr were analyzed by liquid chromatography coupled to mass spectrometry (LC-MS) as previously described (Jain et al., 2012).
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This may take place during the unsuccessful attempts of DNA ligases to seal DNA breaks during incubations in H2O2-free media despite being produced at equal frequency.
Gene transcript levels between different experimental conditions were compared to bacterial strains grown in standard LB media at 37°C with aeration at equal cell density (OD600) and were normalized to the 5S rRNA levels.
All strains were homogenously spotted onto solid media (Additional file 1: Figure S2) at equal cellular concentrations (OD750 = 0.2).
As shown in Fig. 7, ocpB was transcribed at equal levels in all media.
Tesla stock was previously rated at "equal weight".
Both media generated equal signals for IL-8.
"Host communities are at equal risk".
20) At equal performance levels.
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