Sentence examples for media as specified from inspiring English sources

CD34+ cells were thawed, counted, assessed for viability and seeded at 0.5 to 2×105 cells/ml in various culture media (as specified in each experiment).

Aliquots of [3H]taurine-loaded synaptosomes (∼0.2 0.3 mg protein) were injected in glass tubes containing 4.5 mL of HEPES-buffered basal, low [NaCl] hypoosmotic, or low [NaCl] isoosmotic media, as specified in the Results section.

RAW 264.7 monocytes were seeded on top of collagen-coated polycarbonate tissue culture inserts (8 µm pore diameter, Nunc), which were placed into well plates with tumor-conditioned media or control media as specified above.

The HEK001 was cultured in a specific keratinocyte serum-free media as specified by the ATCC.

For plaque assays, fivefold drug dilutions were prepared by using growth media as specified below.

Cells were cultured in differentiation media as specified by the manufacturer (R&D, Minneapolis, MN, USA; CCM005-008, and CCM011), MesenPRO complete media without differentiation supplements, and GBM CM for 21 days.

Cells were reverse transfected with siRNA duplex complexed with Lipofectamine RNAiMAX (Invitrogen) reagent in serum free RPMI1640 media without phenol red (Invitrogen) as specified by manufacturer's instruction.

Luria Bertani agar and M9 Minimal Media broth was prepared in the laboratory as specified in the Handbook of Microbiological Media [ 17].

Zinc depletion and abundance were obtained by supplementing media with ethylenediamine tetraacetic acid (EDTA) or ZnCl2, respectively, as specified in each experiment.

Briefly, samples of 100 μl of conditioned media were incubated on ELISA plates at 4°C overnight, followed by incubations with detection reagents as specified in the manufacturer's protocol.

The maximum wave speed is known as soon as the media are specified, while the minimum spatial stepsizes are known after the configuration has been discretized.