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Exact(7)
The appropriate amino acids were added to minimal media as required.
Bacto agar (Difco; 1.5% w/v) was used to make plates from these media, as required.
The appropriate amino-acid stocks were added to minimal media as required.
Cultures were supplemented with media as required, and fresh media was added 12 h prior to experimental treatment.
Antibiotics (Sigma) were added to culture media as required at the following concentrations: ampicillin, 50 μg/mL for E. coli; spectinomycin, 100 μg/mL for S. agalactiae, 50 μg/mL for E. coli.
Chloramphenicol (30 mg/L), Tetracycline (25 mg/L) or ampicillin (100 mg/L) was added to the media as required to select for the corresponding markers in the bacterial chromosome or plasmid.
Similar(53)
As does hearing her described in the media as requiring "reassurance all the time that she was hot and sexy," or feeling desperate "to stay young and skinny to remain attractive to her husband".
Skim occasionally as required.
Do as required.
Make changes as required.
Adjust as required.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com