Sentence examples for media and substrate from inspiring English sources
Each enzyme discussed responds differently to high pressure depending on the pressure range, temperature, source, solvent or media, and substrate.
We show that each of the steps (propagation of ESC→generation of neural stem cells (NSC →induction of dopaminergic precursors→maturation of dopaminergic neurons) could utilize xeno-free defined media and substrate, and that cells could be stored at intermediate stages in the process without losing their functional ability.
Work presented here indicates that skin keratinocytes are able to proliferate at a higher calcium concentration, as long as suitable media and substrate conditions are present.
All chemicals for media and substrates were obtained from Sigma-Aldrich Canada Co. (Oakville, ON, Canada).
Due to its non-ionic nature, agarose as aqueous gel has been widely used as culture media and substrates for electrophoresis [3, 4].
All chemicals, media, and other substrates were purchased from Sigma-Aldrich (St . Louis MO) unless otherwise noted.
In experiments on fermentation balance NaHCO3 was omitted from the media and insoluble substrates were separated from cells by centrifugation of the cultures in 50 ml tubes for 60 s at 1.700 g.
Typical in vitro expansion of hESCs involves either direct coculture with mitotically inactivated mouse (or human) embryonic fibroblasts (MEFs) or feeder-free methods, where preconditioned media and biological substrates such as Matrigel are employed [ 1, 6].
HTM cells incubated in serum-free media and on nonadhesive substrate generated free-floating spheres that could be grown for more than 3 months.
These methods have become well established through the development of specialized serum free media formulations, selective culture and substrate modifications which allow for successful routine cultivation of keratinocytes [ 13].
It has been observed in several tissues that direct isolation of cells in serum-free media and on nonadhesive substrates results in the formation of spherical clusters of cells known as free-floating spheres.
