The NCS will combine direct measures of environmental media and biological specimens with indirect questionnaire measures (e.g., health care access, utilization, and quality; health status, age, sex, income, education) and extant demographic, geographic, and environmental data (Needham et al. 2005).
B. pseudomallei was isolated from one specimen (0.4%) using standard media and from two specimens (0.8%) using the enhanced protocol.
Each swab was inserted into a separate vial containing viral transport media and kept refrigerated; specimens were delivered in batches to the laboratory weekly.
Stool specimens were aseptically inoculated using selective media and species isolation was further processed following standard procedures.
Previously described methods [24] were optimized for the instrument, cells, and media specimens.
Harvests of drops were also made from T. × media and L. × marschlinsii specimens on the campus of the University of Victoria, Victoria, British Columbia.
†Liver abscess, pleural fluid, tracheal culture after tracheostomy placement, surgical drain fluid culture, eye culture from eye with conjunctivitis, cultures from other eye specimens, culture from soft tissue of the neck, culture from ear with otitis media, and cultures from other ear specimens.
Similarly, the laboratory receiving the specimen may be able to attempt cell culture isolation only after preparing cells and media and be unable to store the specimen in the meantime.
The MCZ provides guidelines for researchers to facilitate the efficient intake of specimen information, digital media, and other research data into the museum database, MCZbase.
The time between stool collection and inoculation of transport media needed to be not more than 6 hours, and the time between placing the specimen in transport media and accession was not more than 18 hours.
