Sentence examples for media and maintained from inspiring English sources

Cells were stained with PI for at least 30 min and run on the Imagestream X. Cell culture conditions and growth media were as previously described by Moreno et al.24 PN1 (wild-type haploid, strain 972h− mating type, lab stock) cells were grown in YE4S media and maintained in exponential phase.

Following 1 hour of treatment, the CPT was washed out, cultures were resuspended in fresh SC-Leu media, and maintained at permissive temperature for an additional 6 hours.

Upon growth to confluency, cells were pipet scratched, cultured in serum-free media and maintained in hypoxia for 24 hours [29].

Cells were transferred to serum free media and maintained at 0.1% O2 in a humidified hypoxic chamber prior to collecting lysates and blotting with a HIF-1α antibody (Fig. 4A top panel).

To determine whether a continual supply of ES cells could be achieved using a fed-batch approach over a specified time frame, shake flasks were inoculated with 1×104 vc/mL in 25 ml media and maintained at 140 rpm at over a 16 d period.

The cells were re-suspended in capacitating media and maintained at 37°C for 4 h (5% CO2).

In a market saturated with similarly positioned brands, we felt the most effective and memorable way to craft our unique story was to leverage social media and maintain a 24/7 "on" voice for the brand.

To prevent acidification of the culture media and maintain tissue viability, intestinal IVOCs require a hyperoxic gas environment [4 6].

In addition to managing the cell bank, the facility makes culture media and maintains a resale storeroom for laboratory products.

They say top pressures include exams, social media and maintaining friendships at a time when hormones and emotions can run high.

Mop.com will be spun off and will not be included in Renren's IPO, but its survival and profitability are evidence of Chen's team's strength in monetizing social media and maintaining growth.