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Inoculant concentration was determined by spread-plating on NFb (Hungria and Araujo 1994; Döbereiner et al. 1995) and RC (Cassán et al. 2010) solid media and adjusted to the concentration of 2 × 108 CFU (colony forming units) mL−1 in all three crop seasons.
Sorted cells were further washed and resuspended in RPMI-10 media and adjusted to 107 cells/ml.
After one wash with PBS, cells were resuspended in culture media and adjusted to a concentration of 3 × 10 cells ml−1.
For cytokine secretion assays, PBMC were thawed in warm media and adjusted to a final concentration of 2 × 10/ml in RPMI-1640 medium supplemented with 10% v/v heat-inactivated fetal calf serum, 2 mM glutamine, penicillin/streptomycin and 20 mM HEPES.
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Embracing the concept of digitalised media (and adjusting your product accordingly) is certainly seen as an increasingly pragmatic idea.
This is useful for news publishers like Yahoo and Fox Sports, who can see what content is actually resonating on social media and adjust their editorial strategies accordingly.
The improved quantitative knowledge derived from this data may help to design more efficient artificial insemination strategies, for example, by optimising the viscosity and chemical composition of fertilisation media and adjusting injection techniques to maximise upstream swimming of sperm cells.
Harvested cells were washed and re-suspended in Dulbecco's complete media, counted and adjusted to 106 cell/ml.
Freshly grown adherent RAW 246.7 or 23ScCr (TLR4-deficient) macrophages were harvested, washed and re-suspended in Dulbecco's complete media, counted and adjusted to 106 cell/ml.
The bioluminescence induction assay was conducted as follows: E. coli K12 MG1655 soxS::lux or E. coli K12 MG1655 katG::lux were washed from the cultivation media and then adjusted with 0.85% NaCl in water to obtain a final optical density at 545 nm of 0.1; a 50 μL aliquot of the culture was then added to each well filled with a GO suspension as described above.
The stocks were then diluted in the appropriate media containing 10% serum and adjusted to the correct concentration.
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