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Increased levels of p42 ERK phosphorylation were observed in Panc1 cells treated with 10 ng ml−1 of Sema5A-ECD for 5 and 30 min compared with cells treated with media alone as well as a slight increase in the level of p44 ERK phosphorylation.
PHA (10 µg/ml) was included as positive control and media alone as negative control.
Streptokinase/Streptodornase and PHA were used in all assays as positive controls and cells and media alone as the negative control.
After four hours of incubation, WT MHEC stimulated with degranulated mast cell preparation from WT mice expressed significantly higher mRNA levels of VCAM-1, ICAM-1, P-selectin, and E-selectin than those treated with culture media alone, as determined by RT-PCR (Figure 1A 1D).
Pookweed mitogen (Gibco, Auckland, NZ) diluted at 1/1000 will be used as a positive control and media alone as negative control.
Whole blood (125 μl, diluted 1 : 10 with warm RPMI 1640) was incubated with 5 × 10 CFU/ml BCG vaccine, or 1 μg/ml Staphylococcal enterotoxin B or media alone as positive and negative controls, respectively, in 24-well plates at 37°C, 5% CO2, 80% humidity for 6 days, following a method adapted from Soares et al.[ 33].
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For instance, trucker's exposure to mid-media alone as compared to those who were not exposed to any intervention reported significantly higher consistent condom use with paid female partners.
Interventions using social media, alone or as part of a complex intervention, were included.
Interventions using social media, alone or as part of a complex intervention, were included based on Kaplan and Haenlein's 8 classifications.
As the media alone contain proteins (as shown in Figure 5a c), we performed western blot analysis on the fractions corresponding to the different peaks found by size exclusion chromatography to identify the light-chain species observed in cell culture.
Cells with media alone were used as negative control and activated cells as positive control.
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