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Here we describe how recent adaptations of fluorescence-based solution kinetic methods can be used to determine practically all important mechanistic parameters of NA-based motor proteins.
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Using Hertzian relationships and a finite element model of a cylinder-on-flat contact configuration, a set of critical fretting fatigue experiments have been conducted to determine the relative effects of six local mechanistic parameters on fretting fatigue life.
This is a very typical finding in dynamic models in that predictions can be robust even when inferences of exact combinations of mechanistic parameters may not always be possible from model fits [ 28].
Nevertheless, for immobilized protein solutions and tissues, calculation of mechanistic parameters using BPP-type models with their inherent assumptions about the nature of the local interactions causing relaxation and the shape of the spectral density function is likely erroneous.
In this vein, it should be emphasised that the mechanisms underlying the development of each cancer vary, and a consideration of the mechanistic parameters mirroring the different molecular pathways is required for tailored cancer therapy.
The existence of oscillatory behavior depends on the conditions of the mechanistic parameters in the feedback loop - conditions that cannot always be generalized analytically and therefore, must be treated by numerical analysis.
All subsequent experiments evaluating mechanistic parameters utilized a rotenone concentration of 50 nM.
The oscillatory behavior (if and when it exists) is purely governed by the values of the system mechanistic parameters.
The analysis shows that the existence or absence of oscillatory behavior is dictated by the numerical values of the individual mechanistic parameters.
In the subgroup of hypertensives, quercetin was capable of decreasing 24 h SBP, suggesting a cardioprotective effect of quercetin, but without effects on mechanistic parameters.
Aim of this study was to pinpoint mechanistic parameters occurring during egfr copy number gains leading to a stable EGFR overexpression and high sensitivity to extracellular signalling.
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