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The current work focuses on a mechanistic characterization of polyglutamine homodimerization as a function of chain length and temperature.
Test results presented herein demonstrate an original approach to the mechanistic characterization of pavement foundation systems which is of interest to engineers using mechanistic-empirical pavement design methods.
The methods outlined here enable mechanistic characterization of the RNA-guided DNA cleavage activity of Cas9 and may assist in further development of the enzyme for genetic engineering applications.
The current lack of thorough understanding and mechanistic characterization of the interlayer bonding behavior that enables to accurately simulate the change in structural responses of pavement system is probably the greatest deficiency in our ability to properly evaluate the effect of interlayer bonding condition for asphalt pavements.
In a mechanistic characterization of this reaction presented elsewhere, we demonstrate that both vertebrate isoforms of Unc45 behave as activators of the Hsp90 dependent folding of the smooth muscle myosin motor domain [24].
Mechanistic characterization of variants identified by genetic studies is the key to understanding molecular disease mechanisms.
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The structural, biochemical, and cell-based studies reported here have laid a foundation for further mechanistic characterizations of the SREBP and SCAP proteins in fission yeast, as well as higher organisms.
These protocols are applicable to mechanistic characterization and engineered exploitation of other tailoring enzymes that act on CP-tethered substrates in natural product biosynthesis and structural diversification.
In contrast to the rapidly expanding identification of individual lncRNAs, mechanistic characterization has lagged behind.
Though androgens alter muscle function and composition, this area of research lacks mechanistic characterization and several researchers believe that androgens act through other anabolic pathways like insulin like growth factor-1 to promote muscle growth[36].
The testing strategy needs to reach beyond the primary screening of toxicants to document adverse effects; additional mechanistic characterization in secondary assays and whole-genome transcriptomics analysis of hits is an essential part of the strategy.
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