Until recently, reliable methods did not exist for accessing biological systems broadly at their mechanistic (molecular and cellular) levels, for purposes of not only understanding them but of altering or designing them to carry out desired useful functions.
As metabolites represent the final outcome of gene expression and activity, the metabolome can be perceived as the ultimate readout of the biochemical and physiological state of a cell, that is, a direct link between mechanistic biochemistry and cellular phenotype 11.
In contemporary terms, this is equivalent to the important claim that our fully mechanistic organismic, molecular, and cellular branches of biology all constitutively require the intentional stance.
We take a multidisciplinary approach to tackle these problems that includes computational modeling, in vitro biochemistry and biophysics, genome-wide screening, and mechanistic cellular biochemistry.
We are distinguished from Psychology and Cognitive Science by emphasizing a mechanistic, molecular, cellular and circuit-level understanding of behavior and disease.
Micromolar hits taken from high-throughput screening were optimized for biochemical and cellular mechanistic potency to ∼10 nM, as exemplified by compound 12az.
Comprehensive gene expression profiling and proteomic analyses have enabled a deeper molecular and cellular mechanistic understanding of post-injury brain regeneration.
In spite of this apparent limitation, both methods can provide a structural framework for a mechanistic understanding of molecular and cellular functions.
The purpose of this study was to establish an experimental human lung cell model for PMMTM carcinogenesis studies that would allow further molecular and cellular mechanistic studies underlying cancer-like phenotypes.
Interest in the toxicological aspects of oxidative stress has grown in recent years, and research has become increasingly focused on the mechanistic aspects of oxidative damage and cellular responses in biological systems.
