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Most structural and mechanistic analyses of the intron have focused on domains 1 and 5, which contain the residues essential for catalysis, and on domain 6, which contains the branch-point adenosine.
These studies, which indicated that FAs are molecularly dynamic sites, have mostly estimated a single FRAP halftime, without providing detailed mechanistic analyses of the processes involved.
Hence, the present account is not as liberal as the previous mechanistic analyses of the Watt governor (Bechtel 1998).
These enable in-depth mechanistic analyses of the therapeutic effects of a given treatment, but might also be useful in cases where longer-term treatment is not feasible.
Further mechanistic analyses of occupancy will be performed in more biophysically focused follow-up studies that however are beyond the current scope of this work.
This study presents a basis for future mechanistic analyses of TORC1 control and CLS in fission yeast and will help identify new proteins functioning in these fundamental processes.
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Our dataset has expanded over nine fold the known number of mouse VR alleles, and will enable mechanistic analyses into the genetics of innate behavioural differences in mice.
To increase the power of our mechanistic analyses, we planned to randomise 144 of these to RO-DBT.
Unexpectedly, extensive mechanistic analyses revealed no evidence of direct binding to Tie2, nor direct activation of Tie2 phosphorylation.
These kinds of mechanistic analyses are critical for predictive toxicology using gene arrays.
Mechanistic analyses revealed that inhibition of aurora kinases induced polyploidy and the ATM/Chk2 DNA damage response, which mediated senescence and a NF-κB-related, senescence-associated secretory phenotype (SASP).
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