Paired pulse facilitation was significantly reduced in both structures of aged NEP-deficient mice, suggesting the involvement of presynaptic mechanisms (data not shown).
Of note, DEX treatment alone (0.1 μM) did not suppress the basal activity of the CRH-promoter, indicating that basal CRH drive is not governed by CREB/CRE dependent mechanisms (data not shown).
Sequence analysis revealed no classic CpG island in the promoter region of LOC554202, suggesting that this host gene is not regulated primarily via DNA methylation mechanisms (data not shown).
Non-specific dye transfer due to endocytosis and other membrane opening mechanisms are temperature dependent [40]; dye uptake experiments carried out at 4°C show no perceptible difference in dye uptake validating the absence of these non-specific mechanisms (data not shown).
Simulated intermediate (and not directly observable) outcomes were consistent with risk factor assumptions, with CRC-specific risk factors affecting only their associated mechanisms (data not shown).
Interestingly, in the postnatal cortex of the Nogo-A KO mice, we did not detect any major abnormalities, although a surprisingly high interindividual variability in the cell density of layers II IV neurons was observed, perhaps pointing to incomplete compensatory mechanisms (data not shown).
