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To better understand the mechanism of reprogramming inhibition, we next set out to determine the point at which the inhibitory signal acts to prevent stem cell formation.
However, before they can be used in a clinical setting, the mechanism of reprogramming needs to be better understood.
Consistent with this view, overcoming senescence enhances both iPS reprogramming and neural conversion, implicating a general mechanism of reprogramming where overcoming senescence may generate and develop complete and functional lineages.
In addition, as we discuss below, dissecting miRNA targets could also provide insights on the mechanism of reprogramming process.
The mechanism of reprogramming is not clear.
The present study is the first to evaluate the properties of individual SECNT embryos using transcriptomic profiles an approach that can help decipher the mechanism of reprogramming.
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We are also interested in understanding the molecular mechanisms of reprogramming by somatic cell nuclear transfer (SCNT).
Hamasaki, M. et al. Pathogenic mutation of ALK2 inhibits induced pluripotent stem cell reprogramming and maintenance: mechanisms of reprogramming and strategy for drug identification.
The key objectives in studying reprogramming include enhancing reprogramming efficiency, understanding the molecular mechanisms of reprogramming, and more importantly improving the quality of iPSCs, which is important for their potential therapeutic applications.
Comparison analysis between the SSEA3-positive and negative populations may help us elucidate the currently poorly understood mechanisms of reprogramming.
Experiments involving manipulation of anti-proliferative pathways [19], [20], [21], [22] offer insights into the mechanisms of reprogramming and may significantly augment reprogramming efficiencies.
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