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The monolayer was mechanically wounded using a sterile 10 μl pipette tip.
Saplings were mechanically wounded using a drill machine equipped with a 3-mm-diameter drill bit.
Rosette leaves of eight-week-old plants were mechanically wounded using forceps [ 27].
Scratch-assays, where a monolayer of cells is mechanically wounded using a pipet tip, are traditionally used for epithelial cell wounding (Sholley et al., 1977).
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Mechanically wounded plants were used for these experiments because wounding initiates an instantaneous and synchronous response that results in the generation of similar VOC profiles as those produced by aphid infestation.
The RNA isolated from leaves of plants that were infested with beet armyworm (1), infested with water weevil (2), mechanically wounded (3), or unwounded (4) was used.
GLVs collections were performed on ∼2.2 g of either non wounded or mechanically wounded 5 week-old Arabidopsis plants in ∼4 L glass desiccators-style containers (Duran Inc., Germany).
The total RNA isolated from beet armyworm-infested plants, water weevil-infested plants, mechanically wounded plants, and untreated control plants was used for the construction of MPSS libraries.
Total RNAs from roots, stems, leaves, seeds, flowers (petals, stamens and pistils), petals at different stages, herbivore-infested leaves, mechanically wounded leaves, and hormone-treated leaves were extracted using Trizol following the manufacturer's instructions (Invitrogen, Carlsbad, CA, USA).
The system was used to monitor the emissions from in vitro mechanically wounded ivy (Hedera helix) and of in vitro grown tomato plants (Lycopersicon esculentum Mill).
The mechanically wounded plants that were punched with a brush leaving 0.5 1 mm holes spaced 2 mm apart were used a positive control.
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