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To investigate the effect of STC1 on injury-induced calcium modulation, confluent monolayers of lung epithelial cells (A549) were mechanically stimulated to initiate a calcium wave.
Likewise, spins that do not receive the original excitation may in fact move into the imaging plane after the RF excitation pulse, and since they were not quantum mechanically stimulated to begin with, no MR-signal may be returned.
When cortical astrocytes were mechanically stimulated to raise [Ca2+]cyt, this stimulus also caused large increases in [Na+]cyt.
When cortical astrocytes were mechanically stimulated to raise [Ca2+]cyt, inhibition of the NCX with benzamil resulted in a significant reduction in peak and cumulative Ca2+cyt accumulation.
Tenocytes were mechanically stimulated to test the hypothesis that rest insertion compared to continuous stretching initially up-regulates SCXA and TGFB1 mRNA levels [ 22– 24] and is accompanied by enhancement of gene expression and protein levels of pro-collagen type I and III, whereas low (100) and high (1000) cycle numbers would have equivalent effects on these same variables.
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NMB-1 (1 µM) was applied to neurons mechanically stimulated at two intensities, 9 and 12 µm, which were then tested for capsaicin sensitivity (Fig. 4a).
In contrast to flies mechanically stimulated at night, both male and female flies that were stimulated in the same manner during the day, when they would normally be active, showed increased sleep relative to controls (Figure 2C).
We divided flies into three treatment groups: mechanically stimulated at night (Night Mechanical Stimulus), mechanically stimulated during the day (Day Mechanical Stimulus), and un-stimulated controls (see Figure 1).
The cells were mechanically stimulated at around 7% strain with 1 cycle per minute for 2 days.
When saliva is mechanically stimulated due to the large volume of water present in this type of saliva, would produce lower proteins concentration when compared with a resting saliva sample [27, 36].
Perhaps as in basal conditions, a decrease in [Na+]cyt that was recorded from mechanically stimulated astrocytes exposed to benzamil could be due to increased activity of NKA, although another alternative is possible (see below).
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