Sentence examples for mechanically scraped using from inspiring English sources

Then the whole corneal and limbal epithelium was mechanically scraped using a surgical blade.

For HIF-1/2α immunoblots, plates were transferred immediately to ice, rinsed with ice-cold PBS containing complete protease inhibitor cocktail and mechanically scraped using 50 100 μl RIPA buffer containing protease inhibitor cocktail.

For lancet scrapings, lesion materials were superficially scraped using a scalpel and 2% xylocaine, and scraped material was placed into a vial containing 70% alcohol.

The specimen was taken out and dried, and the film was scraped using a non-metallic scrapper.

∼2000 colonies were obtained and scraped using 5 ml EMM-Leu.

Freshly grown 1536 density arrays grown on YPD at 25° were scraped using sterile water.

The OSE layer was mechanically scraped several times using a sterile surgical blade Swann-Morton, Sheffield, United Kingdomm, ref. 0501), which was washed in the surrounding culture medium to retrieve a scraped suspension of cells to set up cell cultures.

Powder of porous silicon was then mechanically scraped from the substrate [4, 14].

The substrate-bound NRAs were mechanically scraped, sonicated in ethanol, and deposited on carbon-coated copper grids for TEM characterization.

Porous silicon powders were then mechanically scraped from the etched wafers, and after ultrasonic pulverization (band ultrasonic finger of 2.5 mm in diameter) and optional filtering by 1-μm syringe filters, the dispersions in methanol, water or PBS were obtained.

One ml of CelLytic (Sigma-Aldrich Ltd) was added and incubated during 15 min at room temperature, and then the cells were mechanically scraped.