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Clots were mechanically released from the walls of the tubes and centrifuged at 3500 g for 8 min.
After 2 days of incubation, the attached FPCL were mechanically released from the sides of the culture plates.
Lattices were mechanically released from the well by gentle addition of 1 ml of BMe in each well.
After 2 days of culture the attached FPCLs were mechanically released from the sides of the culture plates.
Following 2 days of attached-culture FPCLs were mechanically released from the sides of the culture dishes and digital images of the contracting gels collected over a 5 day incubation period.
For attached-matrix experiments, harvested FPCLs were treated as described above except that they were not mechanically released from the sides of the dishes throughout the 5 day incubation period.
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Therefore, laser-tweezer-traction should transmit deep inside the cells to mechanically release Ca2+ from ER. Cytoskeleton is known to transmit mechanical forces and conduct mechanotransduction (Hamill and Martinac, 2001; Orr et al., 2006; Schwartz and DeSimone, 2008), so we investigated the role of cytoskeleton and its associated proteins in the force-induced ER Ca2+ release.
However, since the ordered molecular crystals from which these nanofibres are built are only weakly van der Waals bound; they are mechanically instable if released from the growth substrate, and thus, further manipulation (transfer [6] and integration) becomes extraordinarily difficult.
Hence, it was concluded that ATP released from mechanically stimulated cells was a principal mediator responsible for the rise in intracellular calcium in ligament cells.
Extracellular ATP released from mechanically injured cells is an important stimulus for MV production [1], but this response is usually limited spatially as well as temporally by the rapid breakdown of extracellular ATP by ectoATPases.
Incubating astrocytes with KB-R7943 (30 μM; 10 min) reduced the peak and cumulative amount of glutamate released from mechanically stimulated astrocytes, indicating that Ca2+ entry through NCX is important for the elevation of cytoplasmic [Ca2+]cyt necessary for mechanically induced Ca2+-dependent glutamate release from astrocytes.
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