Sentence examples for mechanically lysed using from inspiring English sources

Cell pellets were mechanically lysed using a Dounce homogeniser in a detergent-free hypotonic buffer.

The samples were then mechanically lysed using a bead beater (Mini-Beadbeater, Biospec Products, Bartlesville, USA) two times for 5 minutes each.

Cells were mechanically lysed using silica beads and a bead-beater (FastPrep, QBiogene, Carlsbad, CA, USA) for eight cycles of 30 s pulses each with a 30 s interval on ice.

Frozen cells were thawed and resuspended in 10 mM HEPES buffer (pH 7.5) containing 500 mM sodium chloride, 5% glycerol, 2 mM β-mercaptoethanol, and supplemented with 5 mM imidazole, and mechanically lysed using a microfluidizer (Microfluidics, model M-110EH) at 1,000 bar pressure.

Cells were resuspended in Tel-Test RNA-Bee (Friendswood, TX) and mechanically lysed using a bead beater (BioSpec Products, Bartlesville, OK) as described [ 32].

Cells were isolated by centrifugation (6000 g, 15 min), resuspended in lysis buffer (50 mM potassium phosphate, 300 mM NaCl, 10 mM imidazole, pH 7.4), mechanically lysed using a Constant Systems cell disrupter (20 kpsi) and the lysate cleared by centrifugation (30,000 g, 45 min).

Splenocytes were mechanically dissociated by disruption through steel mesh, and red blood cells were lysed using a hypotonic ammonium chloride solution (82.9 g ammonium chloride, 10 g potassium bicarbonate, 0.37 g EDTA, 1 l sterile water for a 10× solution).

Erythrocytes were lysed using a lysing solution (lysing buffer; BD Pharm Lyse™ BD Biosciencess, Japan) to segregate leukocytes.

Cells were lysed using RIPA buffer supplemented with protease inhibitors.

Cells were lysed using NP40 lysis buffer supplemented with PMSF and protease inhibitor cocktail.

Then, red blood cells were lysed using ACK lysis buffer (Sigma, St . Louis MO, USA).